Making a dilution series

Aim: To make a dilution series to investigate the concentration

Equipment: A potassium permanganate crystal, six large test tubes, tweezers, a plastic transfer pipette, a test tube rack, 10ml measuring cylinder

Method:

1. Place the six test tubes in a test rack. Label the rack with numbers 1-6

2.  Using the measuring cylinder, fill the test tube 1 with 10 ml of water. Fill the remaining test tubes with 5ml of water

3. Using your tweezers, add a single crystal of potassium permanganate to test tube 1

4. Gently shake the test tube until the crystal has dissolved

5. Using the transfer pipette, carefully remove exactly 5ml from the test tube 1 and pour in into test tube 2

6. Rinse the transfer pipette again and repeat the transfer process for test tube 4, 5 and 6

 Discussion:  We put a tiny bit of solute into solvent to make a solution and to make a dilution series.